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Yyyyyy x. yyyyyy

0000 xxxxxx xxxx , xxxx , xxxxx 00000

xxx-xxx-xxxx (c)

281-657-4788 (h)

abc@xyz.com

Country of Citizenship: United States

SSN: xxx-xx-xxxx

 

Vacancy Posting: INSERT POSTING NUMBER

Position: Research Biologist

Location: Atlanta, GA

 

 

Core Competencies

 

Research Biological Science, Health Science, Interdisciplinary Science, Scientific Research, Research Methodologies Development/Implementation, Clinical/Pre-clinical Studies, Project Management, Program Administration, Strategic Planning, Analysis, Scientific Publication, Scientific/Technical Reporting, Presentations, Regulatory Compliance, Quality Assurance, Standard Operating Procedures (SOP) Development, Training & Development, Staff/Lab Supervision, Classroom Instruction, Consulting, Contracting, Contractor Management, Cross-Functional Collaboration, Communications, Grant Proposals, Documentation, Administrative Management, Biotechnology, Pharmaceuticals, Virology, Gene Therapy, Vaccine Development, Immunology, Clinical Devices, Molecular Biology, Recombinant DNA Technology, Cell Culture, Cancer Progression/Tumorigenesis, Animal Science, Information Synthesis, Public Health, Medical Practices, Safety
 

Professional Experience

 

11/2007-Present

Inovio Biomedical corporation

The Woodlands, TX

Annual Salary: $74K/annual USD

Hours Worked per Week: 40

Supervisor Name: Amir Khan; Phone: 281-296-7300x215; Permission to Contact: Yes

Research Scientist

 

Successfully develop biological assays supporting preclinical studies focused on DNA vaccine development.  Proficiently analyze data and present findings to senior staff. Efficiently prepare complex manuscripts and grant proposals supporting objectives.  Resourcefully develop software supporting electroporation device for enhanced DNA vaccine delivery. Effectively conduct and thoroughly analyze in vivo DNA delivery experiments for optimization of conditions. 

 

Contribute dynamic leadership skills toward managing and collaborating with cross-functional team members spanning in-house quality assurance, regulatory consultant, design house, manufacturing facility, and research contract facilities, among others, in facilitating projects related to smallpox, HPV and electroporation device. Have maximized productivity, efficiency and quality by developing and executing proactive standard operating procedures (SOPs) for DNA vaccine delivery clinical devices.

 

 

Page 1 of 5

Yyyyyy x. yyyyyy                                                                                                                  Page 2 of 5

 

07/2006-11/2007

Baylor College of Medicine, Breast Center

Houston, TX

Annual Salary: $45K/annual USD

Hours Worked per Week: 40

Supervisor Name: Mike Lewis; Phone: Confidential; Permission to Contact: Confidential

Postdoctoral Fellow

Applied subject matter expertise and effective communication skills toward delivering weekly presentations of data at Breast Center seminars and weekly lab meetings.  Generated critical resources for Center studies by preparing and writing quality postdoctoral grant fellowships.

 

Focusing on lentivirus projects, successfully designed lentivirus expression vectors using traditional cloning methods and Gateway technology (Invitrogen) as well as encoding shRNAs against Hedgehog genes; additionally designed biochemical assays to test efficacy of overexpression and shRNA lentivirus constructs.  Created innovative strategy to make conditional knockout of Hedgehog gene, Patched-1.

Effectively analyzed whole mammary glands and histological sections for disrupted phenotypes. Designed assays targeted toward monitoring stem cell population in mouse mammary gland.

 

01/2004-07/2006

Baylor College of Medicine

Houston, TX

Annual Salary: $36K/annual USD

Hours Worked per Week: 40

Supervisor Name: Xiaoliu Zhang; Phone: 713-798-1256; Permission to Contact: Yes

Postdoctoral Fellow

 

Contributed decisive leadership skills toward coordinating and supervising productive, quality team of lab technicians.  Actively participated in weekly seminars focused on cell and gene therapy.

 

Strategically developed expression vectors and tested DNA vaccine efficacy in mice against HSV; designed DNA replicon crucial to efficient vaccine development and gene delivery applications. Successfully developed oncolytic HSVs to treat tumors and conducted in vitro and in vivo investigations of antitumor activity. Additionally developed cellular-based therapy involving fusion of macrophages and engraftment to treat Hemophilia A.  Partnered with Amaxa Biosystems (Gaithersburg, MD) in developing transfection protocols for cell lines and primary cells. 

 

08/1999-12/2004

University of Arkansas

Fayetteville, AR

Annual Salary: $13K/annual USD

Hours Worked per Week: 40

Supervisor Name: Mark Parcells; Phone: 302/831-0114; Permission to Contact: Yes

Ph.D. Graduate Research Assistant

 

Working in BSL-3 facility, trained and collaborated with personnel; additionally trained and worked with graduate students in molecular biology techniques. Relied upon to effectively present research findings at major scientific conferences/meetings.

 

Clearly identified and characterized functioning of putative proteins from Marek s Disease Virus ( -herpesvirus).  Transcriptionally-mapped genes for analysis of reactivation from latency. Proficiently genetically engineered expression cell lines for growth kinetic studies.  Efficiently constructed  recombinant MDVs for functional analysis studies. Conducted flow cytometry to study MHC class I surface expression.  Cultured/prepared MDV-infected melanocytes for electron microscopy to effectively evaluate replication cycle.

Yyyyyy x. yyyyyy                                                                                                                  Page 3 of 5


07/1997-07/1999

University of Arkansas for Medical Sciences

Little Rock, AR

Annual Salary: $24K/annual USD

Hours Worked per Week: 40

Supervisor Name: Joan McEwen; Phone: Unknown; Permission to Contact: Yes

Research Technologist II

 

Cloned genes into expression plasmids and tested interaction using Yeast Two-Hybrid system.

Thoroughly screened genomic DNA library for interacting partners. Manually sequenced cDNA encoding alternative oxidase from Histoplasma capsulatum. Successfully defined RNA expression levels for Adenine Nucleotide Translocase genes involved in apoptosis.

 

08/1994-12/1996

West Virginia University

Morgantown, WV

Annual Salary: $9K/annual USD

Hours Worked per Week: 40

Supervisor Name: John Killefer; Phone: 217-333-8482; Permission to Contact: Yes

M.S. Graduate Research Assistant

 

Selected to contribute subject matter expertise and dynamic communication and leadership skills toward teaching undergraduate Biochemistry class for two years. Concurrently steered diverse research projects ranging from screening cDNA library for calpastatin to purifying and characterizing a novel activator of  calpains from skeletal muscle.

 

Specialized Experience/Key Projects

 

Virology/gene therapy/Vaccine development

Transcriptional expression of herpesvirus genes involved in latent and lytic infection and maintenance of transformation.  Immune responses to viral infections.  Isolation and characterization of viral genes.  Developed numerous herpesvirus recombinants and lentivirus-based expression vectors.  Oncolytic HSVs and their use in the treatment of cancer.  Vector designs for vaccine development purposes.  In vivo DNA delivery using electroporation.  Biological assays for measuring expression and immune responses. 

 

Molecular Biology/Recombinant DNA Technology

Over 15 years of experience. Constructed numerous expression constructs. Southern, Northern, and Western blotting, DNA (genomic and plasmid) and RNA isolation (total and mRNA), PCR, RT-PCR, 3 RACE, cloning (all types), DNA transfections (liposome-based and electroporation), ELISA assay and development, BACs, RNA transcriptional mapping, protein purification (affinity and non-specific), flow cytometry, Yeast Two-Hybrid system, protein and nucleic acid quantification, library screening, and immunostaining of cells, and herpesvirus and lentivirus-based expression vectors. 

 

Cell Culture

Eight years of animal cell culture (many cell lines and primary cells).  Construction of stable cell lines.  Isolation and culturing of various mouse primary cells (hepatocytes, macrophages, hematopoietic stem cells [HSC], mesenchymal stem cells [MSC], mammary epithelial cells, and mammary stem/progenitor cells).  Culturing, purification, and isolation of herpesviruses.  Differentiation of bone marrow cells to HSCs, MSCs, and macrophages.  Efficient DNA transfections of HSCs, MSCs, macrophages, and hepatocytes.

 

 

Yyyyyy x. yyyyyy                                                                                                                  Page 4 of 5

 

Specialized Experience/Key Projects continued

 

Animal Experience

Mouse mammary gland isolation, analysis, and histology, BSL-3 experience, HSV vaccine studies, perfusions, blood, plasma, and serum collections, isolation of splenocytes, bone marrow cells, and hepatocytes. 

 

Cancer Progression/Tumorigenesis

Signaling pathways involved in mammary gland and Breast Cancer development (Hedgehog and familiar with ER, EGFR, and Wnt), stem/progenitor cell population assays, proliferation assays, and transgenic and knockout mouse models for development and cancer progression.


Education

 

Postdoctoral Fellow, Breast Cancer, Baylor College of Medicine-Houston, TX; 2006 - 2007

 

Postdoctoral Fellow, Gene Therapy, Baylor College of Medicine-Houston, TX; 2004 - 2006

 

Ph.D., Molecular Virology, University of Arkansas at Fayetteville-Fayetteville, AR; 2004

GPA: 3.7/4.0 scale

 

M.S., Molecular Biology, West Virginia University-Morgantown, WV; 1996

GPA: 3.6/4.0 scale

 

B.S., Animal and Vet. Sci. , West Virginia University-Morgantown, WV; 1994


Professional Affiliations

 

Member, AAAS; 2005

Baylor College of Medicine Postdoctoral Association

(Executive Committee Member) 2004-2007

 

Poultry Science Graduate Association

(Treasurer) 2002-2003

 

Chairman, Poultry Science Graduate Association

(Social Committee) 2001-2002

Member, American Society of Gene Therapy; 2005-2006

 

Awards & Certificates

 

Dr. Richard Ginsberg DNA Vaccine Award, Best Overall Poster at Annual DNA Vaccine Meeting; 2008

Institutional NIH NRSA Fellowship; 2005, 2006

 

P.P. Levine Award, Most Outstanding Publication in the Journal of Avian Diseases; 2004

 

Certificate of Excellence, Annual Poultry Science Meeting for Most Outstanding Graduate Student Poster; 1996

 

Dependent Employee Scholarship, for Undergraduate Education; 1992-1994

 

 

 

 

Yyyyyy x. yyyyyy                                                                                                                  Page 5 of 5

 

Publications

 

Moraes, R.C., Chang, H., Harrington, N., Landua, J.D., Prigge, J.T., Lane, T.F., Wainwright, B.J., Hamel, P.A., and Mike T. Lewis.  Ptch1 is required locally for mammary gland morphogenesis and systemically for ductal elongation.  Development 136:1423-1432 (2009).

Fu, X., Tao, L., Prigge, J., and Xiaoliu Zhang.  A mutant HSV-2 deleted for the PK domain of the ICP10 gene is a potent oncolytic virus.  Mol. Ther. 13:882-890 (2006).

Santin, E.R., Shamblin, C.E., Prigge, J.T., Arumugaswami, V., Dienglewicz, R.L., and Mark S. Parcells.  Examination of the effect of a naturally occurring mutation in glycoprotein L on Marek s disease virus pathogenesis.  Avian Diseases 50:96-103 (2006).

Prigge, J.T., Majerciak, V., Hunt, H.D., Dienglewicz, R.L., and Mark S. Parcells.  Construction and characterization of Marek s Disease Viruses (MDVs) having green fluorescent protein expression tied directly or indirectly to phosphoprotein 38 (pp38) expressions.  Avian Diseases 48:471-487 (2004).

Prigge, J.T. Characterization and functional analysis of the Marek s Disease Virus phosphoprotein 38 (pp38) gene family.  Ph.D. dissertation.  University of Arkansas at Fayetteville (2004).

Parcells, M.S., Arumugaswami, V., Prigge, J.T., Pandya, K., and Robert L. Dienglewicz.  Marek s Disease Virus Reactivation from latency:  Changes in gene expression at the origin of replication.  Poultry Science 82:893-898 (2003). 

Johnson, C.H., Prigge, J.T., Warren, A.D., and Joan E. McEwen.  Characterization of an alternative oxidase activity of Histoplasma capsulatum.  Yeast 20:381-388 (2003).

Prigge, J.T. and Mark S. Parcells.  Regulation of Marek s Disease Virus reactivation and/or lytic infection.  Pre-proposal submitted to U.S. Poultry and Egg (2002).

Prigge, J.T., Kirkpatrick-Keller, D.C., and J. Killefer.  Isolation and characterization of a calpain activator in chicken skeletal muscle.  Poultry Science 77:1411-1416 (1998).  

Prigge, J.T.  Role of calpain system in regulation of muscle growth and meat tenderness in broilers.  M.S. thesis.  West Virginia University (1996).

Patents

US Patent Application No. 12/473634, pending; entitled, Smallpox DNA Vaccine and the Antigens Therein That Elicit an Immune Response.   Authors: R. Draghia-Akli, J. Prigge, N. Sardesai, D. Weiner, L. Hirao, C. Guron, and M. McSorley.

Technology Skills

 

Molecular Biology Software:

Clone Manager, DNASTAR, GCG Wisconsin Package, HIBIO DNASIS/PROSIS

 

Operating Systems:

DOS, MS-Windows 3.X, 95, 98, ME, NT, 2000, XP, Mac OS 7.0/8.0

 

Software Applications:

Adobe Photoshop, MS-Word, MS-PowerPoint, MS-Excel, Corel WordPerfect, Corel Presentations,

Corel Quattro Pro

 

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