jing zhang, ph.d                                                  0000 xxxxxx xxxx , xxxx , xxxxx 00000

(xxx-xxx-xxxx

abc@xyz.com

 

objective

cancer biology ph.d. with expertise in chemical and biological effects of substances by toxicity and abundance eager to contribute toward innovative solutions in research and development for scientific commercial, medical and industrial applications.

 

career profile

ø  bachelor of science, pharmacology.

ø  extensive biological and biochemical knowledge and skills.

ø  well equipped to aid advanced cancer biology research and development of innovative therapeutic agents.

ø  facilitate ectopic and orthotopic mouse models for prostate, colon, breast, liver and pancreatic cancers.

ø  solid understanding and experience in animal ectopic and orthotopic tumor models and treatment of animals with putative new theoretic agents.

ø  oversee evaluation of drug efficacy and toxicity and processing/analysis of tumor tissues.

ø  adept in mammalian cell culture including different cell lines and primary cultures.

ø  perform mouse surgery and administration of drug treatment through tail vain injection and oral gavage.

ø  comprehensive knowledge of mammalian cell culture, molecular biology, cell biology and cancer cell signal transduction.

ø  skilled in isolation of mouse liver cells by local perfusion, and culture of primary hepatocytes.

ø  broad knowledge of immunohistochemistry, immunofluorescent staining, flow cytometry and in-situ hybridization.

ø  assess in vivo effect of advanced and innovative therapeutic agents.

ø  conduct cell biology assays including cell proliferation, apoptosis, migration, invasion and colony formation ability.

ø  wide understanding of molecular biology including dna, rna analysis, rnai construction, transfection and infection and transient/stable cell line generation.

ø  coordinate new technology, strategic planning and alignment of major projects with organizational objectives.

ø  extensive publications (see below).

 

personal traits

ø  proficient in managing project independently and excellent team player collaborating with notable scientists.

ø  proven capacity to effectively develop functional and technical specifications.

ø  highly developed evaluative, analytical and assessment skill sets.

ø  skilled team leader, trainer and mentor.

ø  outstanding communications and interfacing capacity.

professional experience

burnham institute for medical research, la jolla, ca                                                         2007 - present

postdoctoral research associate, program of signal transduction

       recruited to conduct in-depth investigations regarding mechanism of liver tumor development in a mouse model with liver specific deletion of the dna damaged binding protein ddb1.

       researched signaling pathways regulating cell cycle involving ddb1-cul4a ubiquitin ligase and cyclind1.

       developed and perfected techniques of mouse liver perfusion, liver cell isolation, in vitro primary cell culture and isolation of liver progenitor population.

       evaluated function of hepatitis b virus x protein in ddb1-cul4a ubiquitin ligase mediated substrate degradation.

 

 

jing zhang, ph.d

page two

 

the university of texas m.d. anderson cancer center, houston, tx                                        2002 -2007

postdoctoral fellow, department of cancer biology (2007)                                                                                  

       advanced to conduct intracellular signaling transduction using biochemical and molecular biology techniques.

       proved for first time that afap-110 affects ability of protein kinase c to activate p38mapk.

       assessed effect of small molecule inhibitor of non-receptor tyrosine kinase src on growth and lymph node metastasis of prostate cancer in orthotopic nude mouse model.

       facilitated multiple techniques in varying types of cancer nude mouse models and mouse oral gavage of pre-clinical drugs to junior graduate students and postdoctoral fellows.

       collaborated with colleagues within m.d. anderson cancer center and notable outside scientists.

 

graduate research assistant (2002-2007)

       recruited to research and provide first evidence that adaptor protein and src substrate, actin filament-associated protein, afap-110 act as potential biomarkers in human prostate cancer.

       successfully characterized expression of afap-110 in prostate carcinoma tissue microarrays using immunohistochemistry and in human tumor cell lines by immunoblotting.

       effectively initiated shrna expression vectors while developing stable prostate tumor cell clones with decreased afap-110.

       demonstrated that decreased expression of afap-110 reduced tumor incidence and tumor growth in orthotopic nude mouse models.

       investigated role of receptor tyrosine kinase c-met and non-receptor tyrosine kinase src in tumor growth, angiogenesis and metastasis in multiple tumor mouse models.

 

 

earlier background

prior to 2002, served as research assistant, laboratory of genetics, beijing institute for cancer research, school of oncology, peking university, beijing, china, and undergraduate intern, national laboratory of natural and bionic medicines, school of medicine, peking university, beijing, china.  in these assignments, built outstanding research, anti-tumor methodology, drug analysis, investigative and project management skill sets.

education

university of texas health science center at houston, houston, tx

ph.d., cancer biology, 2007

 

peking university, beijing, china

b.s., pharmacology, 2000

 

honors/scholarships/memberships

       sigma xi thesis award-rice university/texas medical center chapter, may 2007

       sowell-huggins graduate fellowship in cancer research, (full coverage of stipend, benefits and tuition), nov 2005- may 2007

       travel award, university of texas health science center at houston, graduate school of biomedical sciences (gsbs), 2003, 2005, 2007

       john p. mcgovern award for gsbs scientific poster contest, 2nd place, nov 2002

       excellent undergraduate scholarship (full coverage of tuition and fees), peking university, sept1995- jun 2000

       student representative: university of texas system task force on doctoral and postdoctoral education-discussion session, jan 2007

       instructor: texas southern university summer program in prostate cancer research, jun 2006

       organizer and chair: presentation section ii, program of cancer biology student retreat, mar 2005

       associate member: american association for cancer research, 2005

jing zhang, ph.d

page three

 

publications

1)     zhang j, park si, artime mc, summy jm, shah an, bomser ja, dorfleutner a, flynn dc and gallick ge. afap-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal adhesion. journal of clinical investigation, 2007;117(10):2962-73.

2)     zhang j, park si, kim mp, flynn dc and gallick ge. afap-110 modulates pkcalpha-mediated p38mapk activation. submitted, 2008

3)     park si, zhang j, kacy ap, araujo jc, najjar am, volgin ay, gelovani jg, wang z and gallick ge. targeting c-src family kinases inhibits growth and lymph node metastases of prostate cancer in orthotopic nude mouse model. cancer research, 2008; 68(9):3323-3333.

4)     dorfleutner a, stehlik c, zhang j, gallick ge and flynn dc. afap-110 is required for actin stress fiber formation and cell adhesion in mda-mb-231 breast cancer cells. journal of cell physiology 2007; 213(3):740-9.

5)     herynk mh, zhang j, parikh nu and gallick ge. activation of src by c-met overexpression mediates metastatic properties of colorectal carcinoma cells. journal of experimental therapeutics & oncology 2007;6(3):205-17.

6)     park si, shah an, zhang j and gallick ge. regulation of angiogenesis and vascular permeability by src family kinases: opportunities for treatment of solid tumors. expert opinion on therapeutic targets, 2007;11(9):1207-17.

7)     shah an, summy jm, zhang j and gallick ge. development and characterization of gemcitabine-resistant pancreatic tumor cells. annals of surgical oncology, 2007 oct 2. epub ahead of print.

8)     gray mj, zhang j, ellis lm, semenza gl, evans db, watowich ss and gallick ge. hif-1alpha, stat3, cbp/p300 and ref-1/ape are components of a transcriptional complex that regulates src-dependent hypoxia-induced expression of vegf in pancreatic and prostate carcinomas. oncogene 2005;24:3110−3120.

 

abstracts

1)       jing zhang and gary e. gallick. the adaptor protein and src substrate afap-110 regulates prostate tumor cell growth through focal adhesion function. innovative minds in prostate cancer today (impact) meeting, los angeles, ca, 2007.

2)       jing zhang, serk in park, daniel c. flynn and gary e. gallick. the actin filament-associated protein afap-110 is overexpressed and contributes to prostate carcinoma development by regulating pkc-mediated fibronectin expression. american association for caner research 98^th annual meeting, los angeles, ca, 2007.

3)       jing zhang, marlene c. artime, serk in park, daniel c. flynn and gary e. gallick. the actin filament-associated protein afap-110 regulates migration of prostate cancer cells by regulation of tgf beta-mediated expression of integrin receptors. american association for caner research 97^th annual meeting, washington, dc, 2006.

4)       jing zhang and gary e. gallick. constitutive activation of c-met signaling alters e-cadherin-mediated contact inhibition. gordon research conferences-cell contact and adhesion meeting, andover, new hampshire, 2003.

 

conferences

invited speaker, minisymposia, american association for cancer research 0000 xxxxxx xxxx , xxxx , xxxxx 00000 carmel creek road, apt. 302 - san diego, ca 92130

(xxx-xxx-xxxx

abc@xyz.com

 

 

 

cover letter text

 

date

 

 

 

hiring agent name

title

company name

address

city, state   zip code

 

dear                   :

 

i am currently seeking a challenging career opportunity in a cancer biology research capacity and am submitting my resume for your review.  in advance, i thank you for your time and consideration.

 

as demonstrated in the accompanying resume, my professional qualifications include a cancer biology ph.d, a b.s. pharmacology, and prestigious assignments at major research institutions in the u.s. and china.  to complement this background, i possess demonstrated expertise in biological/biochemical formulations, development of advanced therapeutic agents and in-depth knowledge of prostate, colon, breast, liver and pancreatic cancers as well as strong analytical, research, investigative and problem-solving capabilities.  as an employee, you will find me to be an enthusiastic and disciplined team player, committed to supporting you in achieving your objectives through superior performance. 

 

i am confident that i could be a valuable asset to your firm, and look forward to interviewing with you in the near future to further discuss my areas of experience and expertise that would be a contribution to your organization.

 

 

 

 

sincerely,

 

 

jing zhang

 

 

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